DNA

Part:BBa_K596001

Designed by: Ebuzer Kalyoncu   Group: iGEM11_Bilkent_UNAM_Turkey   (2011-07-14)

Nitroreductase optimized for C.reinhardtii

Nitroreductase is a protein and it removes nitro functional group make it less toxic molecules; such as from TNT to monoaminodinitrotoluene(ADNT), 2,4-dinitrotoluene(DNT).Enterobacter cloacae oxygen-insensitive NAD(P)H nitroreductase (nfsI) gene is our basic gene.

Author: C.Bryant at all.

Reference: 1. Bryant C, Hubbard L, McElroy WD. Cloning, nucleotide sequence, and expression of the nitroreductase gene from Enterobacter cloacae. The Journal of biological chemistry. 1991;266(7):4126-30. Available at: http://www.ncbi.nlm.nih.gov/pubmed/1999406.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 661
    Illegal XhoI site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 199
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 618


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Categories
Parameters
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